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5'UTR CGG 99x FMR1
(Plasmid #63089)


Item Catalog # Description Quantity Price (USD)
Plasmid 63089 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    low Amp
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
  • Species
    H. sapiens (human)
  • Entrez Gene
    FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).

This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5'UTR CGG 99x FMR1 was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 63089 ; ; RRID:Addgene_63089)