5'UTR CGG 99x FMR1-FLAG
PurposeContains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with a FLAG tag.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63090||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)low Amp
Growth TemperatureRoom Temperature
Gene/Insert nameexpanded CGG repeats (99 repeats) within the 5'UTR of FMR1
SpeciesH. sapiens (human)
Entrez GeneFMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation). These CGG are in frame with the FLAG tag.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:5'UTR CGG 99x FMR1-FLAG was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 63090 ; http://n2t.net/addgene:63090 ; RRID:Addgene_63090)