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pGEX4T-E2-25K
(Plasmid #63572)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63572 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBE2-25K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    645
  • Mutation
    Isoform 1 (200 resiudes)
  • Entrez Gene
    UBA7 (a.k.a. D8, UBA1B, UBE1L, UBE2)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-E2-25K was a gift from Titia Sixma (Addgene plasmid # 63572 ; http://n2t.net/addgene:63572 ; RRID:Addgene_63572)
  • For your References section:

    SUMO modification of the ubiquitin-conjugating enzyme E2-25K. Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK. Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. 10.1038/nsmb903 PubMed 15723079