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Addgene

pCMV5-Flag-HDAC3
(Plasmid #63676)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63676 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV5-Flag
  • Total vector size (bp) 5974
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDAC3
  • Alt name
    histone deacetylase 3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Hdac3
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-Flag-HDAC3 was a gift from Qingbo Xu & Lingfang Zeng (Addgene plasmid # 63676 ; http://n2t.net/addgene:63676 ; RRID:Addgene_63676)
  • For your References section:

    HDAC3 is crucial in shear- and VEGF-induced stem cell differentiation toward endothelial cells. Zeng L, Xiao Q, Margariti A, Zhang Z, Zampetaki A, Patel S, Capogrossi MC, Hu Y, Xu Q. J Cell Biol. 2006 Sep 25;174(7):1059-69. Epub 2006 Sep 18. 10.1083/jcb.200605113 PubMed 16982804