pCVD002
(Plasmid
#63688)
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Purposefor use in sequencing and mapping of the V. cholerae enterotoxin genes
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBR325
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5996
- Total vector size (bp) 8000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameenterotoxin genes
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SpeciesV. cholerae
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Insert Size (bp)4000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD002 was a gift from James Kaper (Addgene plasmid # 63688 ; http://n2t.net/addgene:63688 ; RRID:Addgene_63688) -
For your References section:
Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin. Lockman H, Kaper JB. J Biol Chem. 1983 Nov 25;258(22):13722-6. PubMed 6315707
Map uploaded by the depositor.
