pCVD315
(Plasmid #63737)

• Purpose: (Empty Backbone) Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF
• Depositing Lab: James Kaper
• Publication: Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Backbone

• Vector backbone: pKO-l
• Backbone size (bp): 3878
• Modifications to backbone: pCVD315 is a derivative of the promoter-screening vector pKO-1 in which bases 1 through 311 have been replaced with the multiple cloning region of M13mpl9RF plus bases 29 through 185 of pBR322. Additional manipulations removed the PstI and AccI restriction sites located outside of the multiple cloning region.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pCVD315 MCS
  • pCVD315 cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo

The copy number of this plasmid construction has increased ca 100 fold from its parent. Reason unknown.

Restriction sites: EcoRI, SstI, KpnI, SmaI/XmaI, BamHI AccI, XbaI, SalI SphI HindIII, ClaI, PstI