PurposeDetect translation initiation from UUG start codon (E. coli lacZ)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63861||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Tetracycline, 10 μg/mL
Copy numberLow Copy
Insert Size (bp)3200
MutationUUG start codon
Entrez GenelacZ (a.k.a. b0344, ECK0341)
- Promoter PtacI
- Cloning method Restriction Enzyme
- 5′ cloning site NaeI (destroyed during cloning)
- 3′ cloning site ScaI (destroyed during cloning)
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
AUG --> UUG start codon in lacZ
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG 414 was a gift from Albert Dahlberg (Addgene plasmid # 63861 ; http://n2t.net/addgene:63861 ; RRID:Addgene_63861)
For your References section:Decoding fidelity at the ribosomal A and P sites: influence of mutations in three different regions of the decoding domain in 16S rRNA. O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE. Nucleic Acids Res. 1997 Mar 15;25(6):1185-93. gka224 [pii] PubMed 9092628