PurposeExpression of beta-galactosidase at high levels in E. coli
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63867||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Tetracycline, 10 μg/mL
Copy numberLow Copy
Insert Size (bp)3200
Entrez GenelacZ (a.k.a. b0344, ECK0341)
- Promoter PtacI
- Cloning method Restriction Enzyme
- 5′ cloning site NaeI (destroyed during cloning)
- 3′ cloning site ScaI (destroyed during cloning)
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pSG25 expresses very high levels of beta-galactosidase in E. coli. Various mutant derivatives are used to measure translational errors.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG 25 was a gift from Albert Dahlberg (Addgene plasmid # 63867 ; http://n2t.net/addgene:63867 ; RRID:Addgene_63867)
For your References section:Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity. O'Connor M, Dahlberg AE. Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8. PubMed 8415679