MSCV-miR-34a
(Plasmid #63932)

• Purpose: MSCV vector for overexpressing mouse miR-34
• Depositing Lab: Lin He
• Publication: Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi: 10.1101/gad.233585.113. Epub 2014 Feb 14.

Backbone

• Vector backbone: pMSCV PIG
• Backbone size w/o insert (bp): 7657
• Total vector size (bp): 8169
• Modifications to backbone: This vector is made by Ross Dickins by deleting a EcoRI site in the original MSCV-PIG vector.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: microRNA 34a
• Species: M. musculus (mouse)
• Insert Size (bp): 519
• GenBank ID: 723848
• Entrez Gene: Mir34a (a.k.a. Mirn34a, mir-34a, mmu-mir-34a)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: MSCV

Resource Information

• Supplemental Documents:
  • MSCV-miR-34a
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry