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(Plasmid #64064)


Item Catalog # Description Quantity Price (USD)
Plasmid 64064 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4707
  • Total vector size (bp) 15606
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Cytoplasmic dynein-2
  • Alt name
    Cytoplasmic dynein 2
  • Alt name
    IFT dynein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Deleted amino acids 1-1090, extra C-terminal valine (V4307) added
  • Entrez Gene
    DYNC2H1 (a.k.a. ATD3, DHC1b, DHC2, DNCH2, DYH1B, SRPS2B, SRTD3, hdhc11)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • His tag (N terminal on insert)
    • ZZ tag (N terminal on insert)
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCCTTCATCCGTTTCCAC
  • 3′ sequencing primer CCTGCTCAACCAGAAGTGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing identified one E to K amino acid residue substitution in the ZZ tag. The depositing laboratory does not expect this residue change to affect tag function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-GFP-dynein-2(D1091–Q4307) was a gift from Andrew Carter (Addgene plasmid # 64064 ; ; RRID:Addgene_64064)
  • For your References section:

    Structure of human cytoplasmic dynein-2 primed for its power stroke. Schmidt H, Zalyte R, Urnavicius L, Carter AP. Nature. 2015 Feb 19;518(7539):435-8. doi: 10.1038/nature14023. Epub 2014 Dec 1. 10.1038/nature14023 PubMed 25470043