OAH2
(Plasmid
#64074)
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PurposeExpression of O-acetyl-L-homoserine sulfhydrylase (OAH2) of Thermus thermophilus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJ411
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Backbone manufacturerDNA 2.0
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameO-acetyl-L-homoserine sulfhydrylase
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Alt nameOAH2
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SpeciesSynthetic; Thermus thermophilus
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Tag
/ Fusion Protein
- none
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For expression of non-tagged oah2 only.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OAH2 was a gift from Vern Schramm (Addgene plasmid # 64074 ; http://n2t.net/addgene:64074 ; RRID:Addgene_64074) -
For your References section:
Chemoenzymatic Synthesis of (36)S Isotopologues of Methionine and S-Adenosyl-l-methionine. Poulin MB, Du Q, Schramm VL. J Org Chem. 2015 May 15;80(10):5344-7. doi: 10.1021/acs.joc.5b00608. Epub 2015 Apr 23. 10.1021/acs.joc.5b00608 PubMed 25884979
