VZV ORF 60 (gL)
(Plasmid
#64082)
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Purposeeukaryotic expression plasmid for VZV glycoprotein L
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCI neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVZV glycoprotein L
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Alt nameVZV ORF 60
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Specieshuman herpesvirus type 3 (varicella zoster virus)
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Insert Size (bp)477
- Promoter CMV IE with intron
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VZV ORF 60 (gL) was a gift from Randall Cohrs (Addgene plasmid # 64082 ; http://n2t.net/addgene:64082 ; RRID:Addgene_64082) -
For your References section:
Human anti-varicella-zoster virus (VZV) recombinant monoclonal antibody produced after Zostavax immunization recognizes the gH/gL complex and neutralizes VZV infection. Birlea M, Owens GP, Eshleman EM, Ritchie A, Traktinskiy I, Bos N, Seitz S, Azarkh Y, Mahalingam R, Gilden D, Cohrs RJ. J Virol. 2013 Jan;87(1):415-21. doi: 10.1128/JVI.02561-12. Epub 2012 Oct 17. 10.1128/JVI.02561-12 PubMed 23077312
