pKMD106h - intein-Cas9(C574)
(Plasmid
#64191)
-
PurposeExpresses intein-Cas9(C574) in mammalian cells
-
Depositing Lab
-
Depositing OrganizationHarvard University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 64191-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 64191-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepJDS246
- Backbone size w/o insert (bp) 3399
- Total vector size (bp) 8850
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Mach1
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namemammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
-
Alt nameintein-Cas9(C574)
-
SpeciesStreptococcus pyogenes
-
Insert Size (bp)5451
- Promoter CMV
-
Tag
/ Fusion Protein
- NLS - 3x FLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer OK569
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byKeith Joung (pJDS246)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 64191-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 64191-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKMD106h - intein-Cas9(C574) was a gift from David Liu (Addgene plasmid # 64191 ; http://n2t.net/addgene:64191 ; RRID:Addgene_64191) -
For your References section:
Small molecule-triggered Cas9 protein with improved genome-editing specificity. Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR. Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6. 10.1038/nchembio.1793 PubMed 25848930
