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hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
(Plasmid #99277)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99277 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MillerLab H2B-mVenus-IRES-mPlum, DNA 2.0 Order #62961, Gene 179575
  • Backbone manufacturer
    DNA 2.0
  • Modifications to backbone
    cut out mVenus-IRES-mPlum with SalI and BamHI and insert mOrange2-IRES-EGFP
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    H2B-mOrange2-IRES-EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2434
  • Promoter EF1 alpha-human
  • Tags / Fusion Proteins
    • mOrange2 (N terminal on insert)
    • H2B (N terminal on insert)
    • EGFP (N terminal on insert)
    • IRES (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Puro
  • Alt name
    Puromycin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    600
  • Promoter PGK-mouse
  • Tag / Fusion Protein
    • PGK (N terminal on backbone)

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    H2B-Venus sequence from Anna-Katerina Hadjantonakis: Addgene plasmid # 60141 (https://www.addgene.org/browse/sequence/60141/) Nowotschin, S., Xenopoulos, P., Schrode, N., & Hadjantonakis, A.-K. (2013). A bright single-cell resolution live imaging reporter of Notch signaling in the mouse. BMC Developmental Biology, 13, 15. doi:10.1186/1471-213X-13-15

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin was a gift from Jordan Miller (Addgene plasmid # 99277 ; http://n2t.net/addgene:99277 ; RRID:Addgene_99277)
  • For your References section:

    Tubulogenesis of co-cultured human iPS-derived endothelial cells and human mesenchymal stem cells in fibrin and gelatin methacrylate gels. Calderon GA, Thai P, Hsu CW, Grigoryan B, Gibson SM, Dickinson ME, Miller JS. Biomater Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h. 10.1039/c7bm00223h PubMed 28661522