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PTEF1-yEGFPCLN2PEST-pRS406
(Plasmid #64406)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64406 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    GFPCLN2PEST-pRS406
  • Backbone manufacturer
    Mateus and Avery 2000
  • Modifications to backbone
    destabilized GFP
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEF1 promoter
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TEF1 (a.k.a. YPR080W)
  • Promoter TEF1 promoter
  • Tag / Fusion Protein
    • yEGFPCLN2PEST (destabilized yeast EGFP) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer yGFP-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PTEF1-yEGFPCLN2PEST-pRS406 was a gift from Claudia Vickers (Addgene plasmid # 64406 ; http://n2t.net/addgene:64406 ; RRID:Addgene_64406)
  • For your References section:

    Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae. Williams TC, Espinosa MI, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Apr 1;14:43. doi: 10.1186/s12934-015-0223-7. 10.1186/s12934-015-0223-7 [pii] PubMed 25886317