Purpose(Empty Backbone) Integrative E. coli/S. cerevisiae shuttle vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64533||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerRobert Gnuegge
- Backbone size (bp) 4023
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Promoter-truncated LEU2d marker gene.
Integrates at Ty1 delta sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRG235 was a gift from Joerg Stelling (Addgene plasmid # 64533 ; http://n2t.net/addgene:64533 ; RRID:Addgene_64533)
For your References section:A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923