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FRP1671
(Plasmid #64538)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64538 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRG216
  • Backbone manufacturer
    Robert Gnuegge
  • Backbone size w/o insert (bp) 4557
  • Total vector size (bp) 6727
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET15
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2266
  • Entrez Gene
    MET17 (a.k.a. YLR303W, MET15, MET25)
  • Promoter (lexA-box)_4-P_CYC1_minimal

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert's promoter was derived from FRP793 (Ottoz et al. [2014]: Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRP1671 was a gift from Joerg Stelling (Addgene plasmid # 64538 ; http://n2t.net/addgene:64538 ; RRID:Addgene_64538)
  • For your References section:

    A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae. Gnugge R, Liphardt T, Rudolf F. Yeast. 2015 Dec 9. doi: 10.1002/yea.3144. 10.1002/yea.3144 PubMed 26647923