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TetO-FUW-NfiA
(Plasmid #64901)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64901 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    TetO-FUW
  • Backbone size w/o insert (bp) 8421
  • Total vector size (bp) 9971
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nfia
  • Alt name
    Ctf
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1558
  • GenBank ID
    18027
  • Entrez Gene
    Nfia (a.k.a. 1110047K16Rik, 9430022M17Rik, CTF, NF1-A, NF1A)
  • Promoter TetO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa (destroyed during cloning)
  • 3′ cloning site Nhe (not destroyed)
  • 5′ sequencing primer GCAGAGCTCGTTTAGTGAACCGTC
  • 3′ sequencing primer AGCAGCGTATCCACATAGCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NfiA coding was kindly Provided by Dr G. Messina
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetO-FUW-NfiA was a gift from Vania Broccoli (Addgene plasmid # 64901 ; http://n2t.net/addgene:64901 ; RRID:Addgene_64901)
  • For your References section:

    Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. Caiazzo M, Giannelli S, Valente P, Lignani G, Carissimo A, Sessa A, Colasante G, Bartolomeo R, Massimino L, Ferroni S, Settembre C, Benfenati F, Broccoli V. Stem Cell Reports. 2015 Jan 13;4(1):25-36. doi: 10.1016/j.stemcr.2014.12.002. Epub 2014 Dec 31. 10.1016/j.stemcr.2014.12.002 PubMed 25556566