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(Plasmid #64973)


Item Catalog # Description Quantity Price (USD)
Plasmid 64973 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6688
  • Total vector size (bp) 7135
  • Modifications to backbone
    Addition of a His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    sortase A
  • Alt name
  • Species
    Staphylococcus aureus ATCC 10832
  • Insert Size (bp)
  • Mutation
    deleted amino acids 1-59
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • Mxe GyrA intein (C terminal on backbone)
    • chitin-binding protein (C terminal on backbone)
    • His6 tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SapI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    S. aureus sortase A is originally cloned from a genomic DNA of S. aureus (ATCC 10832D) in our lab.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH1-SaSrtA was a gift from Teruyuki Nagamune (Addgene plasmid # 64973 ; ; RRID:Addgene_64973)
  • For your References section:

    Design of Ca2+-independent Staphylococcus aureus sortase A mutants. Hirakawa H, Ishikawa S, Nagamune T. Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585. Epub 2012 Jul 4. 10.1002/bit.24585 PubMed 22729808