RhoC FLARE.sc mCer, mVenus - wt
(Plasmid
#65071)
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PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 65071-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 65071-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTriEx
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 7888
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBD, mCerulean, mVenus, RhoC
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Insert Size (bp)2688
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MutationT153M in mVenus
- Promoter CMV, p10
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 65071-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 65071-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RhoC FLARE.sc mCer, mVenus - wt was a gift from Klaus Hahn (Addgene plasmid # 65071 ; http://n2t.net/addgene:65071 ; RRID:Addgene_65071) -
For your References section:
A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells. Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L. PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. PONE-D-13-18183 [pii] PubMed 24224016
Map uploaded by the depositor.
