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pBS-Cx43-FS
(Plasmid #65440)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65440 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression ; T7 in vitro RNA production

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cx43
  • Alt name
    Connexin 43
  • Alt name
    GJA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1210
  • Mutation
    Frameshift (FS) mutation 35delA
  • GenBank ID
    2697
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Digested out with EcoRV from Addgene plasmid #27383
  • Terms and Licenses

Depositor Comments

FrameShift Mutation at EcoNI site of Cx43 leading to out of frame synthesis after 11 amino acid and premature stop at amino acid 100.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-Cx43-FS was a gift from Trond Aasen (Addgene plasmid # 65440 ; http://n2t.net/addgene:65440 ; RRID:Addgene_65440)
  • For your References section:

    Internal translation of the connexin 43 transcript. Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T. Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31. 10.1186/1478-811X-12-31 PubMed 24884945