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PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
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Depositing Lab
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Depositing OrganizationFriedrich Miescher Institute for Biomedical Research
Located in Switzerland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 65853-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 65853-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonephRL-SV40
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3705
- Total vector size (bp) 3583
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Modifications to backboneRenilla Luciferase coding sequence replaced with split puromycin N-acetyltransferase
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Vector typeMammalian Expression, CRISPR, TALEN
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesplit puromycin N-acetyltransferase coding sequence
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Alt namesplit PuroR
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site AatII (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 65853-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 65853-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB1610_pRR-Puro was a gift from Marc Bühler (Addgene plasmid # 65853 ; http://n2t.net/addgene:65853 ; RRID:Addgene_65853) -
For your References section:
Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells. Flemr M, Buhler M. Cell Rep. 2015 Jul 28;12(4):709-16. doi: 10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16. 10.1016/j.celrep.2015.06.051 PubMed 26190102
Map uploaded by the depositor.
