PurposeCarries 3' UTR of CDK2AP1, a miR-21 target
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65862||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies, modified by the Tuschl lab
- Backbone size w/o insert (bp) 2550
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneCDK2AP1 (a.k.a. DOC1, DORC1, ST19, doc-1, p12DOC-1)
- Promoter T7
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR221_CDK2AP1_3UTR was a gift from Thomas Tuschl (Addgene plasmid # 65862 ; http://n2t.net/addgene:65862 ; RRID:Addgene_65862)