pDONR221_ETF1_3UTR
(Plasmid
#65865)
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PurposeCarries 3' UTR of ETF1, an RBPMS target
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies, modified by the Tuschl lab
- Backbone size w/o insert (bp) 2550
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameETF1
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SpeciesH. sapiens (human)
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Insert Size (bp)2210
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Entrez GeneETF1 (a.k.a. D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers used to amplify 3' UTR from human cDNA (HCC1964 cDNA):
ETF1 forward: AAA AAG CAG GCT TCG TAG TCG ACA TGG GTC CGG
ETF1 reverse: AGA AAG CTG GGT GTA TTC TTA AAT ATG GTT TAA TAC
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_ETF1_3UTR was a gift from Thomas Tuschl (Addgene plasmid # 65865 ; http://n2t.net/addgene:65865 ; RRID:Addgene_65865)