p(U6a-BsaI-gRNA)
(Plasmid
#65955)
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Purpose(Empty Backbone) Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBME
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Backbone manufacturerBiomatic
- Backbone size (bp) 2932
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Vector typeInsect Expression
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBsaI cloning site and tracRNA are derived from Addgene DR274.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An online tool to help oligo design for gRNAs is at http://www.averof-lab.org/tools/Tribolium_CRISPR.php
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p(U6a-BsaI-gRNA) was a gift from Michalis Averof (Addgene plasmid # 65955 ; http://n2t.net/addgene:65955 ; RRID:Addgene_65955) -
For your References section:
Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum. Gilles AF, Schinko JB, Averof M. Development. 2015 Jul 9. pii: dev.125054. 10.1242/dev.125054 PubMed 26160901
Map uploaded by the depositor.
