pCVD469
(Plasmid
#66156)
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Purposeexpression of His-tagged espB
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepQE30
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 3461
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameespB
- Promoter T5
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pQE promoter
- 3′ sequencing primer T0term-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
6XHis N-terminal tagged espB pQE30 (Qiagen). Must be grown in strain that carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome (eg XL1-Blue).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD469 was a gift from James Kaper (Addgene plasmid # 66156 ; http://n2t.net/addgene:66156 ; RRID:Addgene_66156) -
For your References section:
Antibodies to intimin and Escherichia coli secreted proteins A and B in patients with enterohemorrhagic Escherichia coli infections. Karpman D, Bekassy ZD, Sjogren AC, Dubois MS, Karmali MA, Mascarenhas M, Jarvis KG, Gansheroff LJ, O'Brien AD, Arbus GS, Kaper JB. Pediatr Nephrol. 2002 Mar;17(3):201-11. 10.1007/s00467-001-0792-z PubMed 11956862