pCVD630
(Plasmid
#66163)
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Purposecloning of ace gene
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCVD315
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Backbone manufacturerKaper lab, Addgene plasmid 63737
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameace
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Alt nameaccessory cholera enterotoxin
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Insert Size (bp)2900
- Promoter Unknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBRforEco
- 3′ sequencing primer MoCloF (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned ace gene from El tor Vibrio cholerae E7946 on a 2.9 Kb EcoRV fragment cloned into pCVD315. Also contains 391 bp of zot.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD630 was a gift from James Kaper (Addgene plasmid # 66163 ; http://n2t.net/addgene:66163 ; RRID:Addgene_66163) -
For your References section:
Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette. Trucksis M, Galen JE, Michalski J, Fasano A, Kaper JB. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5267-71. PubMed 8389476