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pGEX6P1-UBE2S-UBD
(Plasmid #66713)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 66713 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    e2-epf
  • Entrez Gene
    UBE2S (a.k.a. E2-EPF, E2EPF, EPF5)
  • Promoter T7
  • Tag / Fusion Protein
    • GST-3C (N terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    isot
  • Entrez Gene
    USP5 (a.k.a. ISOT)
  • Promoter T7

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1-UBE2S-UBD was a gift from David Komander (Addgene plasmid # 66713 ; http://n2t.net/addgene:66713 ; RRID:Addgene_66713)
  • For your References section:

    Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne. Bremm A, Freund SM, Komander D. Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. Epub 2010 Jul 11. 10.1038/nsmb.1873 PubMed 20622874