Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pDD285 Notes

Addgene Diagnostic Digest with ClaI and SpeI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
66826 with the following enzymes: ClaI single digest, SpeI single digest, and ClaI and SpeI double digest

We observed the expected band patterns-

Single Digest with ClaI: 3267bp and 7194bp

Single Digest with SpeI: 3247bp and 7214bp

Double Digest: 612bp, 632bp, 2635bp, and 6582bp

Addgene Diagnostic Digest with EcoRI and BglII

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
66826 with the following enzymes: BglII single digest, EcoRI-HF single digest, and BglII and EcoRI-HF double digest.

We observed the expected band patterns-

Single digest with either BglII or EcoRI: 10461bp

Double digest cut: 7118bp and 3343bp