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pAVU6+27-F30-2xdBroccoli Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pAVU6+27-F30-2xdBroccoli with the following enzymes: ApaLI and HindIII-HF.

We expected ApaLI to give one band at ~6.4kb; however, our results indicate a second restriction site is present.
HindIII was expected and does give 2 bands at ~5.4 and 0.9kb