pRJPaph-bjGFP
(Plasmid
#67032)
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PurposePlasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI
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Depositing Lab
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Depositing OrganizationETH Zurich (Swiss Federal Institute of Technology)
Located in Switzerland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67032-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67032-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSUP202pol4
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Vector typeConjugative bacterial vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
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SpeciesBradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
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Insert Size (bp)807
Gene/Insert 2
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Gene/Insert namebjGFP
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SpeciesSynthetic
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Insert Size (bp)717
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic gene synthesized by Eurofins Genomics (Ebersberg, Germany)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 67032-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67032-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRJPaph-bjGFP was a gift from Julia Vorholt (Addgene plasmid # 67032 ; http://n2t.net/addgene:67032 ; RRID:Addgene_67032) -
For your References section:
Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoefficiens to analyze host-plant infection and colonization. Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM. Mol Plant Microbe Interact. 2015 Jun 2. 10.1094/MPMI-03-15-0054-TA PubMed 26035130
Map uploaded by the depositor.
