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pRJPaph-HcRed
(Plasmid #67035)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67035 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSUP202pol4
  • Vector type
    Conjugative bacterial vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
  • Species
    Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
  • Insert Size (bp)
    807

Gene/Insert 2

  • Gene/Insert name
    HcRed
  • Species
    Synthetic
  • Insert Size (bp)
    687

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pTE100_HcRed (Schada von Borzyskowski et al., ACS Synth. Biol. 4:430-443, 2015)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRJPaph-HcRed was a gift from Julia Vorholt (Addgene plasmid # 67035 ; http://n2t.net/addgene:67035 ; RRID:Addgene_67035)
  • For your References section:

    Stable fluorescent and enzymatic tagging of Bradyrhizobium diazoefficiens to analyze host-plant infection and colonization. Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM. Mol Plant Microbe Interact. 2015 Jun 2. 10.1094/MPMI-03-15-0054-TA PubMed 26035130