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pCellFree_G03 WAVE 2
(Plasmid #67098)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67098 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WAVE 2
  • Alt name
    Wiskott-Aldrich syndrome protein family member 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    WASF2 (a.k.a. IMD2, SCAR2, WASF4, WAVE2, dJ393P12.2)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

WAVE 2 (WASF2) is deleted in-frame from A320 to P381 relative to NM_006990.5 (iso1)
This mutation is not expected to hinder plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCellFree_G03 WAVE 2 was a gift from Kirill Alexandrov (Addgene plasmid # 67098 ; http://n2t.net/addgene:67098 ; RRID:Addgene_67098)
  • For your References section:

    Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K. Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. 10.1002/bit.25814 PubMed 26301602