PurposeStandard for cell-free expression benchmarking.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67102||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeCell-free expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameRAC-alpha serine/threonine-protein kinase
SpeciesH. sapiens (human)
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter T7
/ Fusion Proteins
- His (N terminal on backbone)
- EGFP (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCellFree_G03 AKT1 was a gift from Kirill Alexandrov (Addgene plasmid # 67102 ; http://n2t.net/addgene:67102 ; RRID:Addgene_67102)
For your References section:Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K. Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. 10.1002/bit.25814 PubMed 26301602