Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #67137)


Item Catalog # Description Quantity Price (USD)
Plasmid 67137 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Cell-free expression
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • EGFP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy


  • Gene/Insert name
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • EGFP (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCellFree_G03 was a gift from Kirill Alexandrov (Addgene plasmid # 67137 ; ; RRID:Addgene_67137)
  • For your References section:

    Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems. Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera D, Gonda TJ, Alexandrov K. J Biotechnol. 2015 Feb 10;195:1-7. doi: 10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19. 10.1016/j.jbiotec.2014.12.006 PubMed 25529348