Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pNUT N6His hTFNG
(Plasmid #67240)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67240 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNUT
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 9500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Methotrexate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human serum transferrin
  • Alt name
    hTF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2136
  • Mutation
    Asn413 Asp, Asn611Asp
  • GenBank ID
    AF28814
  • Entrez Gene
    TF (a.k.a. HEL-S-71p, PRO1557, PRO2086, TFQTL1)
  • Promoter SV40
  • Tag / Fusion Protein
    • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CCTTTGCGCCCGGACTCG
  • 3′ sequencing primer GTGGCAACTTCCAGGGCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The laboratory of Dr. Ross T.A. MacGillivray at the University of British Columbia in Vancouver BC Canada

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNUT N6His hTFNG was a gift from Ross MacGillivray & Anne Mason (Addgene plasmid # 67240 ; http://n2t.net/addgene:67240 ; RRID:Addgene_67240)
  • For your References section:

    Differential effect of a his tag at the N- and C-termini: functional studies with recombinant human serum transferrin. Mason AB, He QY, Halbrooks PJ, Everse SJ, Gumerov DR, Kaltashov IA, Smith VC, Hewitt J, MacGillivray RT. Biochemistry. 2002 Jul 30;41(30):9448-54. bi025927l [pii] PubMed 12135367