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(Plasmid #67240)


Item Catalog # Description Quantity Price (USD)
Plasmid 67240 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 9500
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
    human serum transferrin
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Asn413 Asp, Asn611Asp
  • GenBank ID
  • Entrez Gene
    TF (a.k.a. HEL-S-71p, PRO1557, PRO2086, TFQTL1)
  • Promoter SV40
  • Tag / Fusion Protein
    • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CCTTTGCGCCCGGACTCG
  • 3′ sequencing primer GTGGCAACTTCCAGGGCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The laboratory of Dr. Ross T.A. MacGillivray at the University of British Columbia in Vancouver BC Canada

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNUT N6His hTFNG was a gift from Ross MacGillivray & Anne Mason (Addgene plasmid # 67240 ; ; RRID:Addgene_67240)
  • For your References section:

    Differential effect of a his tag at the N- and C-termini: functional studies with recombinant human serum transferrin. Mason AB, He QY, Halbrooks PJ, Everse SJ, Gumerov DR, Kaltashov IA, Smith VC, Hewitt J, MacGillivray RT. Biochemistry. 2002 Jul 30;41(30):9448-54. bi025927l [pii] PubMed 12135367