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(Plasmid #67242)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 67242 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 7653
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Trypanosoma brucei
  • Insert Size (bp)
  • GenBank ID
  • Promoter p-lac
  • Tags / Fusion Proteins
    • none
    • MBP, TEV (N terminal on backbone)
    • Thrombin, HIS (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer 5'- TGC GTA CTG CGG TGA TCA AC -3'
  • 3′ sequencing primer 5'- CTG CAA GGC GAT TAA GTT GG -3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TbSIR2RP1 was a gift from Cynthia Wolberger (Addgene plasmid # 67242 ; ; RRID:Addgene_67242)
  • For your References section:

    Side chain specificity of ADP-ribosylation by a sirtuin. Fahie K, Hu P, Swatkoski S, Cotter RJ, Zhang Y, Wolberger C. FEBS J. 2009 Dec;276(23):7159-76. doi: 10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6. 10.1111/j.1742-4658.2009.07427.x PubMed 19895577