pDONR221-ARF4-no stop
(Plasmid
#67311)
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PurposehArf4 without stop codon in Gateway Entry vector
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
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Vector typeBacterial Expression ; Gateway Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARF4
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Alt namepRK3s-221
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SpeciesH. sapiens (human)
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MutationV53A, L123P and M134I mutations were unintended PCR-generated artifacts
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Entrez GeneARF4 (a.k.a. ARF2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that pDSHA-ARF4-Myc and pDEST47-ARF4-GFP (Addgene plasmid #s 67350 & 67392) were created from this plasmid and also contain the V53A, L123P and M134I mutations in ARF4. These mutations were unintentionally introduced during PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-ARF4-no stop was a gift from Richard Kahn (Addgene plasmid # 67311 ; http://n2t.net/addgene:67311 ; RRID:Addgene_67311)