PurposeMammalian expression of hArf1 with stop codon (native expression)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67453||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneARF1 (a.k.a. PVNH8)
- Promoter CMV
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that the insert contains its native stop codon and does not produce a fusion protein with GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST47-ARF1 was a gift from Richard Kahn (Addgene plasmid # 67453 ; http://n2t.net/addgene:67453 ; RRID:Addgene_67453)