PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67487||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneDIABLO (a.k.a. DFNA64, SMAC)
- Promoter attenuated CMV
/ Fusion Protein
- HA-eGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV1-SMAC-HA-eGFP was a gift from Richard Kahn (Addgene plasmid # 67487 ; http://n2t.net/addgene:67487 ; RRID:Addgene_67487)