Skip to main content
Holiday Schedule: Addgene will be closed December 23 - December 30. Order processing and shipping will resume on January 2, 2023. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSUPER-ARF5a
(Plasmid #67499)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67499 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsARF5 shRNA
  • Alt name
    pSUPER-ARF5a
  • gRNA/shRNA sequence
    TCTGCTGATGAACTCCAGA
  • Species
    H. sapiens (human)
  • Entrez Gene
    ARF5
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer M13-Fwd
  • 3′ sequencing primer M13-Rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-ARF5a was a gift from Richard Kahn (Addgene plasmid # 67499 ; http://n2t.net/addgene:67499 ; RRID:Addgene_67499)
  • For your References section:

    Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA. Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19. 10.1091/mbc.e04-12-1042 PubMed 16030262