spa-GCaMP6f
(Plasmid
#67558)
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Purposephotoactivatable fluorescent calcium reporter
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67558-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67558-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGP-CMV (pEGFP-N1)
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 3955
- Total vector size (bp) 5308
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namespa-GCaMP6f
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Alt namesuperfolder photoactivatable GCaMP6f
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SpeciesSynthetic
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Insert Size (bp)1353
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MutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196N, L221F, T222S, I250V, F256S, N262T
- Promoter CMV
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Tag
/ Fusion Protein
- HIS-tag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer SV40 reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byderived from Addgene clone #40755, published by Chen TW et al. article: ultrasensitive fluorescent proteins for imaging neuronal activity doi: 10.1038/nature12354
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 67558-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67558-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
spa-GCaMP6f was a gift from Ehud Isacoff (Addgene plasmid # 67558 ; http://n2t.net/addgene:67558 ; RRID:Addgene_67558) -
For your References section:
Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480. 10.1038/nmeth.3480 PubMed 26167640