Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #67563)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 67563 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2897
  • Total vector size (bp) 4250
  • Modifications to backbone
    introduction of BgLII mutation before start codon
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    superfolder photoactivatable GCaMP6f
  • Species
  • Insert Size (bp)
  • Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196N, L221F, T222S, I250V, F256S, N262T
  • Tag / Fusion Protein
    • HIS-tag (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    derived from Addgene clone #40755, published by Chen TW et al. article: ultrasensitive fluorescent proteins for imaging neuronal activity doi: 10.1038/nature12354
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-spa-GCaMP6f was a gift from Ehud Isacoff (Addgene plasmid # 67563 ; ; RRID:Addgene_67563)
  • For your References section:

    Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480. 10.1038/nmeth.3480 PubMed 26167640