GFP-A96 ELP
(Plasmid
#67856)
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PurposeGFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue
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Depositing Lab
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Depositing OrganizationUniversity of Southern California
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 67856-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 67856-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneNT-GFPpcDNA3.1
- Backbone size w/o insert (bp) 5256
- Total vector size (bp) 7556
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Modifications to backboneRefer to plasmid construction paper. (NT-GFPpcDNA3.1 (Invitrogen) was modified to contain a BsgI restriction site downstream of the GFP gene)
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Vector typeMammalian Expression
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP fused to ELP (VPGAG) repeated 96 times
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Alt nameGFP-A96
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
- Promoter T7
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Tag
/ Fusion Protein
- GFP fused to N-terminal of ELP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsgI (unknown if destroyed)
- 3′ cloning site Pme1 (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHrev
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 67856-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 67856-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-A96 ELP was a gift from Andrew MacKay (Addgene plasmid # 67856 ; http://n2t.net/addgene:67856 ; RRID:Addgene_67856)
Map uploaded by the depositor.
