Purpose(Empty Backbone) C-terminal fusion to oxBFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68063||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneoxFP inserted via AgeI/NotI restriction sites, removing EGFP
Vector typeMammalian Expression
- Promoter CMV
/ Fusion Protein
- oxBFP (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
Derived from EBFP2. Changed C48S, C70S and V163A
Brightness 17 (product of extinction coefficient and quantum yield and divided by 1,000).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:oxBFP was a gift from Erik Snapp (Addgene plasmid # 68063 ; http://n2t.net/addgene:68063 ; RRID:Addgene_68063)
For your References section:A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227