Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

oxBFP
(Plasmid #68063)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68063 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    oxFP inserted via AgeI/NotI restriction sites, removing EGFP
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tag / Fusion Protein
    • oxBFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from EBFP2. Changed C48S, C70S and V163A
Excitation/Emission 385/448
Brightness 17 (product of extinction coefficient and quantum yield and divided by 1,000).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    oxBFP was a gift from Erik Snapp (Addgene plasmid # 68063 ; http://n2t.net/addgene:68063 ; RRID:Addgene_68063)
  • For your References section:

    A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227