PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68119||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneNot Available
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
- Promoter rab-3
- Cloning method Gibson Cloning
- 5′ sequencing primer caggaaacagctatgac (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Prab-3::NLS::GCaMP6s was a gift from Andrew Leifer (Addgene plasmid # 68119 ; http://n2t.net/addgene:68119 ; RRID:Addgene_68119)
For your References section:Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans. Nguyen JP, Shipley FB, Linder AN, Plummer GS, Liu M, Setru SU, Shaevitz JW, Leifer AM. Proc Natl Acad Sci U S A. 2015 Dec 28. pii: 201507110. 10.1073/pnas.1507110112 PubMed 26712014
Map uploaded by the depositor.