BAP1-001
(Plasmid #68365)

• Purpose: stable expression of BAP1 in mammalian cells
• Depositing Labs: Emanuela Felley-Bosco, Rolf Stahel
• Publication: Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0864(17)30274-5. doi: 10.1016/j.jtho.2017.03.023.

Backbone

• Vector backbone: pCI-puro
• Backbone manufacturer: Dr. J. Mirkovitch, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
• Backbone size w/o insert (bp): 5224
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BAP1
• Alt name: UCHL2
• Alt name: hucep-6
• Alt name: HUCEP-13
• Species: H. sapiens (human)
• Insert Size (bp): 2190
• GenBank ID: 8314 NM_004656.3
• Entrez Gene: BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: chim-int-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes a 729 aa long protein