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RIPR-bio
(Plasmid #68526)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68526 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6654
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RIPR
  • Alt name
    PVX_095055
  • Mutation
    All NXT/S sites mutated to NXA, where X is any amino acid except Proline
  • Promoter CMV
  • Tag / Fusion Protein
    • rat CD4 d3+4, enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer pMT2-F
  • 3′ sequencing primer rb_glob_PA_term-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RIPR-bio was a gift from Julian Rayner & Gavin Wright (Addgene plasmid # 68526 ; http://n2t.net/addgene:68526 ; RRID:Addgene_68526)
  • For your References section:

    A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals New Vaccine Candidates and Protein-Protein Interactions. Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC. PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 10.1371/journal.pntd.0004264. eCollection 2015 Dec. 10.1371/journal.pntd.0004264 PubMed 26701602