pMDR1-221
(Plasmid
#69481)
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PurposeDeletion Construct
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69481-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69481-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL2 Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5598
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDR1 promoter
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Alt nameABCB1
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SpeciesH. sapiens (human)
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Insert Size (bp)339
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MutationInsert includes MDR1 promoter sequence from -221 to +118
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Entrez GeneABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sma I (unknown if destroyed)
- 3′ cloning site Pvu II (unknown if destroyed)
- 5′ sequencing primer GLprimer1
- 3′ sequencing primer GLprimer2
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69481-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69481-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDR1-221 was a gift from Kathleen Scotto (Addgene plasmid # 69481 ; http://n2t.net/addgene:69481 ; RRID:Addgene_69481) -
For your References section:
Transcriptional regulation of the MDR1 gene by histone acetyltransferase and deacetylase is mediated by NF-Y. Jin S, Scotto KW. Mol Cell Biol. 1998 Jul;18(7):4377-84. PubMed 9632821