This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET23a-Mm/HsUBA52
(Plasmid #69560)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69560 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3626
  • Total vector size (bp) 4013
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UBA52
  • Alt name
    Ubiquitin A-52
  • Alt name
    UBCEP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    387
  • GenBank ID
    NM_001033930.1
  • Entrez Gene
    UBA52 (a.k.a. CEP52, HUBCEP52, L40, RPL40)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Human and mouse UBA52 are identical at protein level.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-Mm/HsUBA52 was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 69560 ; http://n2t.net/addgene:69560 ; RRID:Addgene_69560)
  • For your References section:

    The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors. Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE. Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836. 10.1038/srep12836 PubMed 26235645